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KMID : 0525720180230020084
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Journal of Chitin and Chitosan
2018 Volume.23 No. 2 p.84 ~ p.90
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Improvement in Chitinase Productivity of Paenibacillus sp. Isolate by Gamma Ray-Induced Mutation
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Choi Hyun-Sik
Yoon Ki-Hong
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Abstract
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A bacterial strain capable of hydrolyzing colloidal chitin was isolated from rhizosphere soil of cherry tomato equipment in Gyengsangbuk-do province in Korea. The isolate, named YB-1801, showed biochemical identity of 98.4% with Paenibacillus polymyxa, while the nucleotide sequence of its 16S rDNA showed similarity higher than 99% with P. illinoisensis and P. xylanilyticus. The culture filtrate exhibited maximum chitinase activity at 55oC and pH 6.5. In order to improve the chitinase productivity of Paenibacillus sp. YB-1801, culture suspension containing both vegetative cell and spore was irradiated with 60 Co gamma-rays at doses ranging from 0.25 to 2.0 kGy. Following gamma-irradiation, five mutants were isolated by scoring the transparent halo sizes formed around the colonies grown on LB agar plates containing 0.5% colloidal chitin. One of the mutants increased chitinase productivity by more than two folds in LB broth supplemented with and without colloidal chitin over the parent strain. Colloidal chitin served as inducer for chitinase production in both parent and its mutant strains.
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KEYWORD
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Paenibacillus sp., Chitinase, Gamma-ray, Mutation, Productivity
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